Bgee: Gene Expression Evolution

Expression data retrieved for the gene FBgn0017457: U2af38 - in the anatomical structure: "testis" at the stage: "adult stage" - Drosophila melanogaster

Query parameters Anatomical structure: testis
Stage: adult stage
Gene: FBgn0017457 - U2af38
Data parameters
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Hide | TopAffymetrix

Affymetrix experiment GSE4188

Experiment name Transcription profiling of whole testis from wild type, bgcn- and Os+bgcn- Drosophila [GSE4188]
Source GEO
Experiment description Whole testes were dissected from adult males. RNA from five or six biological replicates were generated and the expression profiles were determined using Affymetrix Drosophila Genechip 1 arrays. Comparisons between the bgcn- and Os+bgcn- groups allowed for the identification of stem cell genes.
Chips
  • Chip ID: GSM95945 - Annotation by Bgee curators: Anatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
    • Probeset ID: 154225_at - Mapped to gene: FBgn0017457 - Detection flag: marginal - Quality: low quality
  • Chip ID: GSM95946 - Annotation by Bgee curators: Anatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
    • Probeset ID: 154225_at - Mapped to gene: FBgn0017457 - Detection flag: absent - Quality: high quality
  • Chip ID: GSM95943 - Annotation by Bgee curators: Anatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
    • Probeset ID: 154225_at - Mapped to gene: FBgn0017457 - Detection flag: absent - Quality: high quality
  • Chip ID: GSM95942 - Annotation by Bgee curators: Anatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
    • Probeset ID: 154225_at - Mapped to gene: FBgn0017457 - Detection flag: marginal - Quality: low quality
  • Chip ID: GSM95944 - Annotation by Bgee curators: Anatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
    • Probeset ID: 154225_at - Mapped to gene: FBgn0017457 - Detection flag: marginal - Quality: low quality

Affymetrix experiment GSE7763

Experiment name Transcription profing of Drosophila tissues in adult and larva [GSE7763]
Source GEO
Experiment description A new online resource (flyatlas.org) provides the most comprehensive view yet of expression in multiple tissues of Drosophila. Meta-analysis of the data reveals that a significant fraction ofthe genome is expressed with great tissue specificity in the adult, demonstrating the need for the functional genomic community to embrace a wider range of functional phenotypes. Well-known developmental genes are often re-used in surprising tissues in the adult, suggesting new functions. The homologues of many human genetic disease loci show selective expression in Drosophila tissues with function analogous to the affected tissues in the cognate human disease, providing a useful filter for potential candidate genes. Experiment Overall Design: FlyAtlas is composed of data covering 8 distinct adult tissues (brain, head, midgut, Malpighian tubules,hindgut, testis, ovary, male accessory glands) so far, together with two larval tissues (tubule, fat body), each run with 4 replicates on Affymetrix Dros Genome 2 chips (with probesets for 18770 transcripts), and compared with a matched whole-fly sample.
Chips
  • Chip ID: GSM188108 - Annotation by Bgee curators: Anatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
    • Probeset ID: 1625231_at - Mapped to gene: FBgn0017457 - Detection flag: present - Quality: high quality
  • Chip ID: GSM188110 - Annotation by Bgee curators: Anatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
    • Probeset ID: 1625231_at - Mapped to gene: FBgn0017457 - Detection flag: present - Quality: high quality
  • Chip ID: GSM188109 - Annotation by Bgee curators: Anatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
    • Probeset ID: 1625231_at - Mapped to gene: FBgn0017457 - Detection flag: present - Quality: high quality
  • Chip ID: GSM188111 - Annotation by Bgee curators: Anatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
    • Probeset ID: 1625231_at - Mapped to gene: FBgn0017457 - Detection flag: present - Quality: high quality

Hide | TopExpressed Sequence tags

EST library 2294

Library name Drosophila melanogaster adult testis library [2294]
Source Unigene
Library description Testes dissected from 1-5 day adult y[*] w[67c1]/Y males raised at 25oC. RNA isolated using Trizol (Life Technologies) and a single round of Poly(A)+ selection using Oligotex (Qiagen). cDNA library constructed using Stratagene ZAP-cDNA syntesis kit. Oligo dT-primed, size fractionated ~1-6 kb, and directionally cloned at EcoRI and XhoI in Uni-ZAP XR. Following a single round of amplification pBlueScript SK phagemids were mass excised. A distribution channel for clones is being sought, but not currently available. Requests for clones cannot be honored.
Annotation by Bgee curatorsAnatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
EST

EST library 7236

Library name AT Drosophila melanogaster adult testes pOTB7 [7236]
Source Unigene
Library description The mRNA for the testis library was made from testes and seminal vessicles hand dissected from 0-3 day old Ore-R males. RNA kindly provided by the lab of Margaret Fuller. Sized fractionated cDNAs were directly ligated into pOTB7. Plasmid cDNA library.
Annotation by Bgee curatorsAnatomical structure ID: FBbt:00004928 - Developmental stage ID: FBdv:00005369
EST